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摘要目的 探讨细胞内钙离子螯合剂1，2-双(2-氨基苯氧基)乙烷-N，N，N，N-四乙酸四(乙酰氧基甲酯)(BAPTA-AM)对人肝癌细胞Huh7增殖、迁移、侵袭、细胞周期、凋亡及细胞内钙离子浓度([Ca2+]i)的影响，分析其在Huh7细胞恶性表型中的作用及机制。方法实验分为Control组、BAPTA-AM处理组(10、20 μmol/L)和无钙培养基组(0Ca2+)。采用KFluor488-EdU法检测细胞增殖；Transwell小室法检测细胞迁移与侵袭；流式细胞术检测细胞周期分布及AnnexinV-FITC/PI双染法检测细胞凋亡；采用比率型钙离子探针Fura-2乙酰氧基甲酯(Fura2-AM)负载及PTI高速离子成像系统实时监测[Ca2+]i。结果 与对照组相比，BAPTA-AM能够显著抑制Huh7细胞的增殖、迁移和侵袭能力(P<0.05或P<0.01)，诱导细胞凋亡(P<0.01)，并导致细胞周期阻滞于G0/G1期，S期细胞比例减少(P<0.01)。同时，BAPTA-AM处理降低了Huh7细胞的[Ca2+]i水平(P<0.01)。0Ca2+组在各项恶性表型检测及[Ca2+]i降低方面，均表现出与BAPTA-AM组相似的效果。结论BAPTA-AM通过螯合细胞内钙离子有效降低[Ca2+]i，从而抑制肝癌细胞的恶性生物学行为。该效应与剥夺胞外钙源的效果相同，提示干扰钙离子稳态是抑制肝癌细胞恶性行为的关键机制之一。
Abstract：Objective To investigate the effects of intracellular calcium chelator O，O'-Bis(2-aminophenyl)ethyleneglycol-N，N，N'，N'-tetraacetic acid， tetraacetoxymethylester (BAPTA-AM) on the proliferation， migration， invasion， cell cycle， apoptosis and intracellular calcium concentration ( [Ca2+]i ) of human hepatocellular carcinoma cell line Huh7， and to analyze its role and mechanism in the malignant phenotype of Huh7 cells. Methods The experiment was divided into control group， BAPTA-AM treatment group (10，20 μmol/L ) and calcium-free medium group (0 Ca2+). Cell proliferation was detected by KFluor488-EdU method. Transwell chamber method was used to detect cell migration and invasion. Cell cycle distribution was detected by flow cytometry and apoptosis was detected by Annexin V-FITC / PI double staining. Fura 2 acetoxymethyl ester(Fura2-AM) loading and PTI high-speed ion imaging system were used to monitor [Ca2+]i in real time. Results Compared with the control group， BAPTA-AM could significantly inhibit the proliferation， migration and invasion of Huh7 cells (P<0.05 or P<0.01)， induce apoptosis (P<0.01)， and lead to cell cycle arrest in G0/G1phase， and the proportion of cells in the S phase was significantly reduced (P<0.01). At the same time， BAPTA-AM treatment significantly reduced the [Ca2+]i of Huh7 cells (P<0.01). 0 Ca2+ group showed the same effect as the BAPTA-AM group in the detection of malignant phenotypes and the decrease of [Ca2+]i。Conclusion BAPTA-AM can effectively reduce [Ca2+]i by chelating intracellular calcium ions， thereby inhibiting malignant biological behavior. This effect is the same as the effect of deprivation of extracellular calcium sources， suggesting that interfering with calcium homeostasis is one of the key mechanisms for inhibiting the malignant behavior of liver cancer cells.
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基本信息:
中图分类号:R735.7
引用信息:
[1]金海,万硕,赵思宇,等.钙离子螯合剂BAPTA-AM抑制肝癌细胞恶性生物学行为的实验研究[J].安徽医科大学学报().
基金信息:
国家自然科学基金项目（编号：82460466、81960507）; 贵州省科技计划项目（编号：黔科合基础-ZK[2024]一般323）
2026-09-01
2026-09-01
2026-09-01