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目的 探究机械压力调控对颅缝硬脑膜细胞（S-DCs）与非颅缝硬脑膜细胞（NS-DCs）迁移、促成骨能力的影响。方法 分离培养5日龄SD大鼠硬脑膜细胞（S-DCs）、非颅缝硬脑膜细胞（NS-DCs）及颅缝间充质干细胞（CS-MSCs）。根据细胞来源及是否施加机械压力（1.07 kPa、24 h）设置S-DCs-Normal、S-DCs-Stress、NS-DCs-Normal、NS-DCs-Stress四种实验条件。采用细胞划痕试验检测硬脑膜细胞迁移能力；收集四种实验条件培养基与CS-MSCs共培养并进行成骨诱导。采用茜素红染色检测钙盐沉积，Western blot检测成骨相关蛋白表达。结果 与Normal组相比，Stress处理后S-DCs和NS-DCs的迁移能力均降低（P<0.05）。与S-DCs相比，NS-DCs在相同处理条件下迁移能力较弱（P<0.05）。条件培养基实验显示，Stress处理后CS-MSCs的成骨能力降低（P<0.05），且NS-DCs来源条件培养基的促成骨作用弱于S-DCs(P<0.05)。Western blot结果与茜素红染色趋势一致，Stress处理及NS-DCs来源均可下调成骨相关蛋白表达（P<0.05）。结论 机械压力可显著抑制硬脑膜细胞迁移及其介导的CS-MSCs成骨分化进程。S-DCs的力学耐受性与成骨调控能力显著优于NS-DCs。
Abstract：Objective To explore the effects of mechanical pressure regulation on the migration and bonepromoting ability of suture dural cells(S-DCs) and non-suture dural cells(NS-DCs).Methods Dural cells(SDCs), non-cranial suture dural cells(NS-DCs) and cranial suture mesenchymal stem cells(CS-MSCs) of 5-dayold SD rats were isolated and cultured. Four experimental conditions, namely S-DCs-Normal, S-DCs-Stress, NSDCs-Normal and NS-DCs-Stress, were set according to the source of the cells and whether mechanical pressure(1. 07 kPa, 24 hours) was applied. The migration ability of dural cells was detected by cell scratch test. Four ex‑perimental condition culture media were collected and co-cultured with CS-MSCs for osteogenic induction. Calcium salt deposition was detected by alizarin red staining, and the expression of osteogenesis-related proteins was de‑tected by Western blot.Results Compared with the Normal group, the migration abilities of both S-DCs and NSDCs decreased after Stress treatment(P<0. 05). Compared with S-DCs, NS-DCs has a weaker migration ability un‑der the same processing conditions(P<0. 05). The conditioned medium experiment showed that Stress treatment reduced the osteogenic ability of CS-MSCs(P<0. 05), and the osteogenic effect of the conditioned medium derived from NS-DCs was weaker than that of S-DCs(P<0. 05). The Western blot results were consistent with the trend of alizarin red staining. Both Stress treatment and NS-DCs sources could down-regulate the expression of osteogenesisrelated proteins(P<0. 05).Conclusion Mechanical stress can significantly inhibit the migration of dural cells and the osteogenic differentiation process of CS-MSCs mediated by it. The mechanical tolerance and osteogenic regulation ability of S-DCs are significantly better than those of NS-DCs.
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基本信息:
DOI：10.19405/j.cnki.issn1000-1492.2026.09.009
中图分类号:R329.2
引用信息:
[1]张海东,申杰,高雅洁,等.机械压力对不同部位硬脑膜细胞迁移及促成骨能力的影响[J].安徽医科大学学报,2026(09):1576-1583.DOI:10.19405/j.cnki.issn1000-1492.2026.09.009.
基金信息:
国家自然科学基金项目(编号:82360188); 国家临床重点专科建设项目(编号:Z155080000004); 内蒙古自然科学基金项目(编号:2025SHZR2536); 内蒙古公立医院科研联合基金科技项目(编号:2024GLLH0504); 内蒙古自治区医师协会临床医学研究和临床新技术推广项目(编号:YSXH2024KYF072); 包头医学院科学研究发展基金项目(编号:BYJJ-GCJH202521)
2026-08-07
2026-08-07
2026-08-07