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目的 探索格氏乳球菌SHAMU-LG6对葡萄球菌的拮抗活性。方法 利用VITEK 2 GP鉴定卡、Microflex LT MALDI-TOF质谱分析仪和16S rDNA扩增测序3种方法共同鉴定菌株种属。牛津杯法抑菌试验检测格氏乳球菌SHAMU-LG6对不同葡萄球菌的拮抗活性；XAD16非离子型大孔树脂吸附、梯度乙醇洗脱和旋转蒸发干燥初步分离纯化抗菌活性成分。结果 格氏乳球菌SHAMU-LG6对耐甲氧西林金黄色葡萄球菌、甲氧西林敏感金黄色葡萄球菌、表皮葡萄球菌、腐生葡萄球菌、路邓葡萄球菌、人葡萄球菌、头状葡萄球菌、沃氏葡萄球菌等均具有拮抗效应，抑菌指数分别为3.3、3.0、4.3、2.0、4.0、3.5、3.8和3.5。格氏乳球菌SHAMU-LG6分泌的抗菌活性成分主要存在于70%和80%乙醇洗脱物中。结论 格氏乳球菌SHAMU-LG6对葡萄球菌具有强拮抗效应，其分泌的抗菌活性成分有望成为新型抗菌药物开发的先导化合物。
Abstract：Objective To investigate the antagonistic activity of Lactococcus garvieae SHAMU-LG6 against Staphylococcus. Methods VITEK 2 GP identification card, Microflex LT MALDI-TOF mass spectrometer and 16S rDNA amplification sequencing were used to identify the strain species. The antagonistic activity of L. garvieae SHAMU-LG6 against different Staphylococcus was detected by Oxford cup method for bacterial inhibition; the antimicrobial active components were preliminarily isolated and purified by adsorption on XAD16 nonionic macroporous resin, gradient ethanol elution and rotary evaporation drying. Results L. garvieae SHAMU-LG6 exhibited potent antagonistic effect against methicillin-resistant Staphylococcus aureus, methicillin-susceptible S. aureus, S. epidermidis, S. saprophyticus, S. lugdunensis, S. hominis, S. capitis and S. warneri, with inhibitory indices of 3.3, 3.0, 4.3, 2.0, 4.0, 3.5, 3.8, and 3.5, respectively. The antimicrobial active components produced by L. garvieae SHAMU-LG6 were mainly present in 70% and 80% ethanol eluates. Conclusion L. garvieae SHAMU-LG6 exhibits a potent antagonistic effect on Staphylococcus, and the antimicrobial active components produced by it are expected to be a lead compound for the development of novel antimicrobial agents.
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基本信息:
DOI：10.19405/j.cnki.issn1000-1492.2025.02.002
中图分类号:R378.11
引用信息:
[1]翁胜男,冷贵云,刘颖,等.格氏乳球菌SHAMU-LG6对葡萄球菌的拮抗活性研究[J].安徽医科大学学报,2025,60(02):195-200.DOI:10.19405/j.cnki.issn1000-1492.2025.02.002.
基金信息:
国家自然科学基金(编号：82102460); 安徽医科大学研究生科研与实践创新项目(编号：YJS20230190);安徽医科大学校科研基金项目(编号：2022xkj197); 安徽医科大学第二附属医院临床研究培育计划项目(编号：2021LCZD13)~~
2025-02-06
2025-02-06
2025-02-06