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目的 利用PtPd@FeⅢ-TCPP■UiO-66纳米材料的类酶活性，实现过氧化氢和葡萄糖的定量检测。方法 采用溶剂热法合成PtPd@FeⅢ-TCPP■UiO-66纳米材料，借助扫描电子显微镜(SEM)、透射电子显微镜(TEM)、X射线衍射(XRD)、傅里叶变换红外光谱(FT-IR)等手段对其进行表征。以3，3'，5，5'-四甲基联苯胺(TMB)为显色底物，设置UiO-66、FeⅢ-TCPP■UiO-66、PtPd@FeⅢ-TCPP■UiO-66及空白对照组开展催化活性对比实验。测定目标物线性检测范围、检测限(LOD)，并开展选择性、稳定性与人血清加标回收实验评价检测性能。结果 经元素掺杂和表面修饰的锆基金属有机框架(UiO-66)表现出增强的类过氧化物酶活性，20 min内实现过氧化氢[线性范围：5.0～80 μmol/L；LOD：0.87 μmol/L]和葡萄糖[线性范围：0.2～3.0 mmol/L；LOD：0.175 mmol/L]的低检测限和宽范围检测。检测平台对常见生物酶和金属离子表现出良好的选择性。过氧化氢和葡萄糖的人血清加标回收率分别为98.06%～99.77%、95.11%～104.61%，相对标准偏差分别为4.3%～4.9%、2.9%～4.1%。结论 基于PtPd@FeⅢ-TCPP■UiO-66纳米酶，建立了一种灵敏、可靠的比色检测平台，并展现出良好的临床生化检测应用前景。
Abstract：Objective To achieve the quantitative detection of hydrogen peroxide (H2O2) and glucose by utilizing the enzyme-like activity of PtPd@FeⅢ-TCPP■UiO-66 nanomaterial. Methods PtPd@FeⅢ-TCPP■UiO-66 nanomaterial was synthesized via a solvothermal method and characterized by techniques such as scanning electron microscopy (SEM)， transmission electron microscopy (TEM)， X-ray diffraction (XRD)， and Fourier transform infrared spectroscopy (FT-IR). Using 3，3'，5，5'-tetramethylbenzidine (TMB) as the chromogenic substrate， groups including UiO‑66， FeⅢ‑TCPP■UiO‑66， PtPd@FeⅢ‑TCPP■UiO‑66 and the blank control were set for comparative experiments of catalytic activity. The linear detection ranges and limits of detection (LOD) of target analytes were determined， and the detection performance was evaluated through selectivity， stability and human‑serum spiked‑recovery experiments. Results Zirconium-based metal-organic framework (UiO-66) modified via element doping and surface modification exhibits enhanced peroxidase-like activity， enabling the detection of hydrogen peroxide (linear range: 5.0～80 μmol/L; LOD: 0.87 μmol/L) and glucose (linear range: 0.2～3.0 mmol/L; LOD: 0.175 mmol/L) with low LOD and wide detection range. The detection platform exhibits excellent selectivity over common biological enzymes and metal ions. The spiked recoveries of hydrogen peroxide and glucose in human serum were 98.06%～99.77% and 95.11%～104.61%， with relative standard deviations (RSD) of 4.3%～4.9% and 2.9%～4.1%， respectively. Conclusion A sensitive and reliable colorimetric detection platform was established based on this nanozyme， showing potential application in clinical biochemical detection.
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基本信息:
中图分类号:O657;TB383.1;R446.1
引用信息:
[1]刘开圆,荆琦淑,潘成英,等.纳米酶在过氧化氢和葡萄糖检测中的应用研究[J].安徽医科大学学报().
基金信息:
国家自然科学基金项目（编号：82360364）; 贵州省卫生健康委科学技术基金项目（编号：gzwkj2025-132）; 贵州省科技计划项目（编号：黔科合基础MS[2025]559）; 全国大学生创新训练计划项目（编号：2024106600563）
2026-08-31
2026-08-31
2026-08-31