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目的探讨野菊花总黄酮(TFC)的抗炎作用及部分机制。方法采用二甲苯诱导的小鼠耳肿胀、角叉菜胶诱导的大鼠足肿胀、棉球诱导的大鼠肉芽肿模型观察TFC的抗炎作用;分别采用放射免疫法(RIA)和反相高效液相色谱法(RP-HPLC)检测TFC对大鼠腹腔巨噬细胞产生前列腺素E2(PGE2)和白三烯B4(LTB4)的影响。结果TFC对二甲苯诱导的小鼠耳肿胀、角叉菜胶诱导的大鼠足爪肿胀、棉球诱导的大鼠肉芽肿具有抑制作用;TFC在0.05~50mg/L范围内能够浓度依赖性地抑制PMФ中PGE2和LTB4的产生。结论TFC具有较强的抗炎作用且可能与其影响PGE2和LTB4的生物合成有关。
Abstract：Objective To investigate the anti-inflammatory effects of total flavonoids of chrysanthemum indicum (TFC) and its partial mechanism. Methods Animal inflammatory models were established by injection of dimethylbenzene for mice ear swelling, carrageenin for rat footpad, cotton pellet wool for rat granuloma respectively. The content of prostaglandin E2(PGE2) and leukotriene B4(LTB4) from peritoneal macrophages(PMФ) were measured by RIA and RP-HPLC respectively. Results TFC significantly inhibited mice ear swelling and rat palm swelling and rat cotton pellet granuloma. It also showed that the content of PGE2 and LTB4 from PMФ were inhibited by TFC in dose-dependent manner. Conclusion The TFC has remarkable anti-inflammatory activity, which may be related to inhibitory effects on PGE2 and LTB4 from PMФ.
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基本信息:
DOI：10.19405/j.cnki.issn1000-1492.2005.05.005
中图分类号:R285
引用信息:
[1]张骏艳,张磊,金涌,程文明,过林,邹宇宏,彭磊,张茜,李俊.野菊花总黄酮抗炎作用及部分机制[J].安徽医科大学学报,2005(05):405-408.DOI:10.19405/j.cnki.issn1000-1492.2005.05.005.
基金信息:
国家重大基础研究前期研究专项(编号:2002CCC02900)
2005-10-30
2005-10-30