| 172 | 1 | 61 |
| 下载次数 | 被引频次 | 阅读次数 |
目的 在多孔钛表面构建表没食子儿茶素没食子酸酯(EGCG)与聚赖氨酸(PLL)酚胺交联涂层整合抗菌物质氯己定(CHX),评价涂层的抗菌性能和生物安全性。方法 将纯钛进行碱热预处理制成多孔钛(pTi组),然后在碱性有氧环境下浸没于EGCG与PLL混合溶液中24 h,从而沉积酚胺交联涂层(EP组),再将其进一步浸没于CHX溶液中24 h,使之与CHX整合(EPC组)。用扫描电镜(SEM)、X射线光电子能谱仪(XPS)和水接触角测量仪进行材料表征；通过体外抗菌实验(抑菌圈法、涂板法、浊度法、活/死细菌染色)评价样品体外抗菌性能；体外细胞相容性实验(MTT法和细胞荧光染色)评价其生物安全性；细菌细胞共培养实验评价在有菌环境中钛片表面的细胞黏附增殖能力。结果 SEM见碱热预处理后的多孔钛表面随着涂层沉积孔隙依次减小；水接触角测量示pTi组随着酚胺交联涂层的沉积和与CHX的整合，水接触角逐渐增加。XPS检测见EPC组Ti、O含量降低，C、N含量增加，并出现Cl2p特征峰；体外抗菌实验表明EPC组对金黄色葡萄球菌和伴放线放线杆菌均有良好抗菌性能，抗菌能力能维持7 d以上；MTT法和细胞荧光染色显示EPC组细胞数量随时间增长呈明显的递增趋势且形态良好；细菌细胞共培养实验表明EPC组在有菌环境中细胞仍可有效增殖。结论 纯钛表面EGCG/PLL酚胺涂层整合CHX具有良好的抗菌性能和生物安全性，且能在有菌环境中发挥抗菌作用的同时保障细胞的黏附、增殖。
Abstract：Objective To construct a phenolamine cross-linked coating composed of epigallocatechin gallate(EGCG) and poly-L-lysine(PLL) on the surface of the porous pure Ti integrated with the antibacterial substance chlorhexidine(CHX), and to study the antibacterial property and biosafety. Methods A porous structure(pTi group) was formed on the surface of titanium samples by alkaline heat treatment. Then the pTi group was immersed in the mixed solution of EGCG and PLL in an alkaline aerobic environment for 24 h to obtain the phenolamine group samples(EP group). Finally, the titanium tablets were further immersed in the aqueous solution of chlorhexidine(CHX) for 24 h to obtain the antibacterial groups(EPC group). Microstructure and properties of the surface were characterized by scanning electron microscopy(SEM), X-ray photoelectron spectrometer(XPS) and water contact angle measuring instrument. The antibacterial properties of coating were observed and evaluated by nephelometry, inhibition zone method and live/dead bacterial staining. The cytocompatibility of the coating was evaluated by MTT method and cell fluorescence staining, and the cell adhesion and proliferation ability in bacterial environment were evaluated by the co-culture of bacteria and cells. Results SEM results showed that the pore size of samples after alkali heat treatment decreased with the deposition of the phenolamine coating. The measurement of water contact angle showed that the contact angle increased significantly with the grafting of coating. The intensity of N1s and C1s peaks increased and Ti2p and O1s peaks decreased detected by XPS tests. The Cl2p peak appeared in EPC group referred to the control group. The antibacterial experiments in vitro showed that the antibacterial samples could perform effective antibacterial effects on Staphylococcus aureus and Aggregatibacter actinomycetem-comitans for at least 7 days. In the biocompatibility experiments, MTT and cell fluorescence staining results showed that the adherent cells had good morphology and proliferative activity. The bacteria-cell co-culture results showed that the EPC groups could provide a good environment for cell proliferation and growth with excellent antibacterial properties. Conclusion Chlorhexidine-grafted phenolamine deposited on porous titanium surface displays an effective antibacterial effect with good biosafety performance, which can play an antibacterial role in the bacterial environment while ensuring cell adhesion and proliferation.
[1] Xu J,Zhang J,Shi Y,et al.Surface modification of biomedical Ti and Ti alloys:a review on current advances[J].Materials(Basel),2022,15 (5):1749.doi:10.3390/ma15051749.
[2] Yuan Z,He Y,Lin C,et al.Antibacterial surface design of biomedical titanium materials for orthopedic applications[J].J Mater Sci Technol,2021,78:51-67.doi:10.1016/j.jmst.2020.10.066.
[3] Janson O,Gururaj S,Palmer S,et al.Titanium surface modification to enhance antibacterial and bioactive properties while retaining biocompatibility[J].Mater Sci Eng C Mater Biol Appl,2019,96:272-9.doi:10.1016/j.msec.2018.11.021.
[4] Solderer A,Kaufmann M,Hofer D,et al.Efficacy of chlorhexidine rinses after periodontal or implant surgery:a systematic review[J].Clin Oral Investig,2019,23(1):21-32.doi:10.1007/s00784-018-2761-y.
[5] Machtei E,Frankenthal S,Levi G,et al.Treatment of peri-implantitis using multiple applications of chlorhexidine chips:a double-blind,randomized multi-centre clinical trial[J].J Clin Periodontol,2012,39(12):1198-205.doi:10.1111/jcpe.12006.
[6] 庞诗梦，夏荣，李趁趁，等.钛表面新型抗菌涂层的生物相容性研究[J].安徽医科大学学报，2022,57(9):1355-99.doi:10.19405/j.cnki.issn1000-1492.2022.09.003.[6] Pang S,Xia R,Li C,et al.Study on the biocompatibility of novel antibacterial coating on titanium[J].Acta Univ Med Anhui,2022,57(9):1355-99.doi:10.19405/j.cnki.issn1000-1492.2022.09.003.
[7] Fürst M,Salvi G,Lang N,et al.Bacterial colonization immediately after installation on oral titanium implants[J].Clin Oral Implants Res,2007,18(4):501-8.doi:10.1111/j.1600-0501.2007.01381.x.
[8] Hofman A,Hees I,Yang J,et al.Bioinspired underwater adhesives by using the supramolecular toolbox[J].Adv Mater,2018,30 (19):e1704640.doi:10.1002/adma.201704640.
[9] Burzio L A,Waite J H.Cross-linking in adhesive quinoproteins:studies with model decapeptides[J].Biochemistry,2000,39(36):11147-53.doi:10.1021/bi0002434.
[10] Waite J H.Mussel adhesion-essential footwork[J].J Exp Biol,2017,220(Pt 4):517-30.doi:10.1242/jeb.134056.
[11] Yang Z,He H,Gao Z,et al.A dopamine-like deposition coatings on the polyethylene terephthalate fibers and its interface adhesion with rubber[J].Surf Interfaces,2024:104485.doi:10.1016/j.surfin.2024.104485.
[12] Almatroodi S A,Almatroudi A,Khan A,et al.Potential therapeutic targets of Epigallocatechin Gallate (EGCG),the most abundant catechin in green tea,and its role in the therapy of various types of cancer[J].Molecules,2020,25 (14):3146.doi:10.3390/molecules 25143146.
[13] Rapp M V,Maier G P,Dobbs H A,et al.Defining the catecholcation synergy for enhanced wet adhesion to mineral surfaces[J].J Am Chem Soc,2016,138 (29):9013-6.doi:10.1021/jacs.6b03453.
[14] Fiorillo L,D'Amico C,Mehta V,et al.Chlorhexidine cytotoxicity on oral behaviors:last 20 years systematic review[J].Oral Oncol Rep,2024,9:100245.doi:10.1016/j.oor.2024.100245.
[15] 丁思勰，洪慧蕾，徐凌寒，等.酚胺交联涂层接枝氯己定改善钛表面抗菌性能研究[J].中国修复重建外科杂志，2022,36 (3):335-42.doi:10.7507/1002-1892.202108095.[15] Ding S,Hong H,Xu L,et al.Chlorhexidine-grafted phenolamine coating to improve antibacterial property of the titanium surface[J].Chin J Reparative Reconstr Surg,2022,36 (3):335-42.doi:10.7507/1002-1892.202108095.
[16] Silva D,Guerra C,Muňoz H,et al.The effect of Staphylococcus aureus on the electrochemical behavior of porous Ti-6Al-4V alloy[J].Bioelectrochemistry,2020,136:107622.doi:10.1016/j.bioelechem.2020.107622.
基本信息:
DOI：10.19405/j.cnki.issn1000-1492.2025.01.015
中图分类号:R783.1
引用信息:
[1]洪慧蕾,徐建光,邱华.多孔钛表面EGCG/PLL酚胺涂层整合氯己定的抗菌性能探究[J].安徽医科大学学报,2025,60(01):109-116.DOI:10.19405/j.cnki.issn1000-1492.2025.01.015.
基金信息:
安徽省自然科学基金(编号：2208085QE136)~~