安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔疾病研究重点实验室;
目的 在多孔钛表面构建表没食子儿茶素没食子酸酯(EGCG)与聚赖氨酸(PLL)酚胺交联涂层整合抗菌物质氯己定(CHX),评价涂层的抗菌性能和生物安全性。方法 将纯钛进行碱热预处理制成多孔钛(pTi组),然后在碱性有氧环境下浸没于EGCG与PLL混合溶液中24 h,从而沉积酚胺交联涂层(EP组),再将其进一步浸没于CHX溶液中24 h,使之与CHX整合(EPC组)。用扫描电镜(SEM)、X射线光电子能谱仪(XPS)和水接触角测量仪进行材料表征；通过体外抗菌实验(抑菌圈法、涂板法、浊度法、活/死细菌染色)评价样品体外抗菌性能；体外细胞相容性实验(MTT法和细胞荧光染色)评价其生物安全性；细菌细胞共培养实验评价在有菌环境中钛片表面的细胞黏附增殖能力。结果 SEM见碱热预处理后的多孔钛表面随着涂层沉积孔隙依次减小；水接触角测量示pTi组随着酚胺交联涂层的沉积和与CHX的整合，水接触角逐渐增加。XPS检测见EPC组Ti、O含量降低，C、N含量增加，并出现Cl2p特征峰；体外抗菌实验表明EPC组对金黄色葡萄球菌和伴放线放线杆菌均有良好抗菌性能，抗菌能力能维持7 d以上；MTT法和细胞荧光染色显示EPC组细胞数量随时间增长呈明显的递增趋势且形态良好；细菌细胞共培养实验表明EPC组在有菌环境中细胞仍可有效增殖。结论 纯钛表面EGCG/PLL酚胺涂层整合CHX具有良好的抗菌性能和生物安全性，且能在有菌环境中发挥抗菌作用的同时保障细胞的黏附、增殖。
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基本信息:
DOI：10.19405/j.cnki.issn1000-1492.2025.01.015
中图分类号:R783.1
引用信息:
[1]洪慧蕾,徐建光,邱华.多孔钛表面EGCG/PLL酚胺涂层整合氯己定的抗菌性能探究[J].安徽医科大学学报,2025,60(01):109-116.DOI:10.19405/j.cnki.issn1000-1492.2025.01.015.
基金信息:
安徽省自然科学基金(编号：2208085QE136)~~