2025 01 v.60 96-101
染色体不稳定相关基因GALNT7对结肠癌细胞HCT116增殖和凋亡的影响
基金项目(Foundation):
国家自然科学基金(编号：81972251)~~
邮箱(Email):
zhaoya@yzu.edu.cn;
DOI:
10.19405/j.cnki.issn1000-1492.2025.01.013
中文作者单位:
扬州大学医学院(转化医学研究院);扬州大学江苏省非编码RNA基础与临床转化重点实验室;
摘要(Abstract):
目的 探究染色体不稳定(CIN)相关基因多肽N-乙酰半乳糖氨基转移酶7(GALNT7)对结肠癌细胞HCT116增殖和凋亡的影响。方法 采用慢病毒感染构建敲低GALNT7的HCT116细胞系，通过染色体滴定验证GALNT7与CIN的相关性，运用活细胞计数检测GALNT7对细胞增殖的影响，采用流式细胞术和Western blot检测GALNT7对细胞周期的影响，采用Caspase-3酶活性测定和Western blot检测GALNT7对细胞凋亡的影响。结果 敲低GALNT7后HCT116细胞染色体核型分布更为分散，CIN程度增高，细胞增殖速度减慢；敲低GALNT7后HCT116细胞发生细胞周期阻滞，并且细胞内P21蛋白上调，CDK6蛋白下调；敲低GALNT7后HCT116细胞Caspase-3酶活性上升，切割型PARP1蛋白和PUMA蛋白表达量升高，BCL-2蛋白表达量下降。结论 GALNT7基因可能通过抑制CIN产生促进结肠癌细胞增殖并抑制其凋亡。
关键词(KeyWords):
染色体不稳定;结肠癌;GALNT7;细胞增殖;细胞周期;细胞凋亡
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基本信息:
DOI：10.19405/j.cnki.issn1000-1492.2025.01.013
中图分类号:R735.35
引用信息:
[1]房晓,赵薇,喻文颖等.染色体不稳定相关基因GALNT7对结肠癌细胞HCT116增殖和凋亡的影响[J].安徽医科大学学报,2025,60(01):96-101.DOI:10.19405/j.cnki.issn1000-1492.2025.01.013.
基金信息:
国家自然科学基金(编号：81972251)~~