锦州医科大学附属第一医院重症医学科;辽西区域呼吸与危重症疾病诊疗中心;锦州医科大学第一临床医学院;锦州医科大学基础医学院;锦州医科大学附属第一医院呼吸内科;
目的 探讨miR-155-5p/沉默信息调节因子1(sirt1)信号通路对白色念珠菌诱导川崎病模型小鼠免疫功能的影响。方法 将C56BL/6小鼠分为对照组、川崎病组、拮抗剂对照组、miR-155-5p拮抗剂组、miR-155-5p拮抗剂+si-NC组、miR-155-5p拮抗剂+si-sirt1组，每组12只。除对照组外，其他组小鼠均通过腹腔注射白色念珠菌水溶物构建川崎病模型。建模成功后进行给药处理，1 d给药1次，持续7 d。qRT-PCR检测冠状动脉中miR-155-5p表达；Western blot检测冠状动脉中sirt1蛋白；HE染色检测冠状动脉的病理变化；检测小鼠胸腺指数和脾指数；流式细胞术检测外周血中辅助性T细胞17(Th17)/调节性T细胞(Treg);ELISA检测小鼠血清中白细胞介素(IL)-17、IL-10水平；验证sirt1与miR-155-5p靶向关系。结果 与对照组比较，川崎病组小鼠冠状动脉中有大量炎性细胞浸润，miR-155-5p表达、Th17比例、Th17/Treg比例、IL-17水平升高，sirt1蛋白表达、胸腺指数、脾指数、Treg比例、IL-10水平降低(均P<0.05);与川崎病组比较，miR-155-5p拮抗剂组小鼠冠状动脉中炎性细胞浸润现象有所缓解，miR-155-5p表达、Th17比例、Th17/Treg比例、IL-17水平降低，sirt1蛋白表达、胸腺指数、脾指数、Treg比例、IL-10水平升高(均P<0.05);si-sirt1减弱了抑制miR-155-5p对川崎病小鼠Th17/Treg平衡的促进作用以及对血管炎症的抑制作用，miR-155-5p靶向调控sirt1。结论 抑制miR-155-5p促进川崎病小鼠Th17/Treg平衡，抑制血管炎症的机制可能与上调sirt1表达有关。
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基本信息:
DOI：10.19405/j.cnki.issn1000-1492.2025.02.017
中图分类号:R725.4;R-332
引用信息:
[1]魏刚,田晶,梁冬雪等.miR-155-5p/sirt1信号通路对白色念珠菌诱导川崎病模型小鼠免疫功能的影响[J].安徽医科大学学报,2025,60(02):307-312+320.DOI:10.19405/j.cnki.issn1000-1492.2025.02.017.
基金信息:
辽宁省教育厅基本科研面上项目(编号：LJKMZ20221229)~~