昆明医科大学基础医学院药理学系;浦瑞生物医药技术有限公司;云南民族大学图书馆技术服务部;
目的 研究四氢姜黄素(THC)和纳米四氢姜黄素(THCN)的抗抑郁作用及机制。方法 将46只雄性ICR小鼠随机分为Con组、脂多糖(LPS)组、THC组、THCN组和舍曲林(SER)组。采用腹腔注射LPS建立小鼠抑郁模型。采用旷场实验和强迫游泳实验评估小鼠的焦虑及抑郁样行为；髓鞘染色法检测长期实验小鼠前额皮层脱髓鞘程度；免疫荧光法检测短期实验小鼠前额皮层和海马组织中胶质纤维酸性蛋白(GFAP)和Toll样受体4(TLR4)表达水平。结果 与Con组相比，长期和短期实验LPS组小鼠焦虑抑郁样行为均增加(P<0.05);长期实验LPS组小鼠脱髓鞘程度增加(P<0.01);短期实验LPS组小鼠GFAP表达减少(P<0.01),TLR4表达增加(P<0.05),THC组小鼠TLR4表达减少(P<0.01),THCN组前额皮层的GFAP表达减少(P<0.01),TLR4表达增加(P<0.05)。与LPS组相比，长期实验THC组小鼠抑郁样行为减少(P<0.05),THCN组和SER组小鼠焦虑抑郁样行为减少(P<0.05),短期实验THC组、THCN组小鼠焦虑抑郁样行为减少(P<0.05);长期实验THC组、THCN组和SER组小鼠脱髓鞘程度均减少(P<0.05);短期实验THC组GFAP表达增加(P<0.05),TLR4表达减少(P<0.05),THCN组GFAP表达增加(P<0.05)。与THC组相比，长期实验THCN组、SER组焦虑样行为减少(P<0.05);短期实验THCN组前额皮层的GFAP表达减少(P<0.05),海马DG区TLR4表达增加(P<0.01)。结论 THC和THCN均能改善脂多糖诱导的小鼠抑郁样行为和脱髓鞘化，THC的抗抑郁机制可能与下调TLR4、增加GFAP有关，THCN的抗抑郁机制可能与增加GFAP有关。
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基本信息:
DOI：10.19405/j.cnki.issn1000-1492.2025.01.011
中图分类号:R285.5
引用信息:
[1]谭慧,李远萍,孟靖源等.四氢姜黄素及其纳米制剂对脂多糖诱导的抑郁小鼠的改善作用[J].安徽医科大学学报,2025,60(01):79-86.DOI:10.19405/j.cnki.issn1000-1492.2025.01.011.
基金信息:
国家自然科学基金(编号：82060650); 云南省科技厅科技计划项目(编号：202405AC350045)~~