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目的 分析由健康人粪便中新分离出的一株动物双歧杆菌B4株的全基因组信息，并探究其益生特性。方法 采用体外实验方法对动物双歧杆菌B4的耐药性、溶血性、耐胃酸特性以及生化特性进行评估，并利用二代+三代测序技术对该菌进行全基因组测序以及基因功能注释。结果 经全基因组测序后显示该动物双歧杆菌B4株基因组大小为1 944 146 bp, GC含量为60.49%,不含质粒，总基因数为1 642个。体外实验分析结果显示，该动物双歧杆菌B4株具有良好的益生特性，包括非溶血性和耐胃酸的特性。同时基因组结果显示，该动物双歧杆菌B4株不具有毒素、致病相关基因，耐药基因少且传播能力不高，具有很高的安全性。通过KEGG、COG、GO等基因注释显示其含有很多生物活性酶，如β-半乳糖苷酶、L-乳酸脱氢酶等益生基因。结论 该动物双歧杆菌B4株具有良好的益生特性，在基因层面上展示了优良的安全性，具有益生特性的基因序列。
Abstract：Objective To investigate whole genome information of a newly isolated Bifidobacterium animalis subsp. lactic B4 strain from healthy human feces was analyzed and its probiotic properties. Methods The antimicrobial resistance, hemolytic, gastric acid tolerance and biochemical characteristics of B. animalis B4 were evaluated by in vitro experiments, and its whole genome was sequentially sequenced and functional annotation was performed by next and three-generation sequencing technology. Results Whole genome sequencing of B. animalis B4 showed that its genome size was 1 944 146 bp, with GC content of 60.49%, no plasmid, and a total of 1 642 genes. The results of in vitro analysis showed that the B. animalis B4 had good probiotic properties, including non-hemolytic and stomach acid resistance. At the same time, the genome results showed that the B. animalis B4 strain did not have toxin and disease-related genes, drug resistance genes were few and the transmission ability was not high, so it had high safety. Gene annotation of KEGG, COG and GO showed that it contained many biological active enzymes, such as β-galactosidase, L-lactate dehydrogenase and other probiotic genes. Conclusion The B. animalis B4 has good probiotic properties, showing excellent safety at the genetic level, with a probiotic gene sequence.
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基本信息:
DOI：10.19405/j.cnki.issn1000-1492.2025.02.010
中图分类号:R371
引用信息:
[1]丁瑞培,刘承忠,史灿灿等.动物双歧杆菌B4株的全基因组测序及其益生特性分析[J].安徽医科大学学报,2025,60(02):255-265.DOI:10.19405/j.cnki.issn1000-1492.2025.02.010.
基金信息:
国家自然科学基金青年基金项目(编号：82302568); 江西省教育厅科学技术研究项目——一般项目(编号：GJJ2203414); 安徽省自然科学基金青年项目(编号：2308085QH283); 安徽省教育厅青年骨干教师境内访学研修资助项目(编号：gxjnfx2023003); 安徽医科大学大学生创新创业项目(编号：202310366054)~~