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目的 探讨富血小板纤维蛋白（PRF）提取液对米非司酮诱导损伤的子宫内膜间质细胞（ESCs）的生物学修复功能及其分子机制。方法 采用米非司酮诱导建立ESCs损伤模型，将细胞分为未处理（Con）组、米非司酮损伤（M）组、米非司酮损伤+PRF-提取液处理（M+P）组及米非司酮损伤+PRF-提取液+生长因子抑制剂处理（M+P+T;M+P+Me;M+P+CJ）组。通过CCK-8法检测细胞活力，细胞划痕实验评估细胞迁移能力；ELISA检测PRF-提取液中血管内皮生长因子（VEGF）、血小板衍生生长因子-BB(PDGF-BB)和转化生长因子-β1(TGF-β1)等生长因子的释放及其规律；RT-qPCR和Western blot分别分析ESCs中凋亡相关因子Bcl-2、Bax和Caspase-3的mRNA与蛋白表达水平。结果 PRF-提取液中VEGF、PDGF-BB和TGF-β1均在2周内持续释放，释放高峰集中在第3～7天；PRF-提取液对米非司酮诱导损伤的ESCs细胞的增殖和迁移均有显著的修复能力（均P<0.000 1）;PRF-提取液能显著上调米非司酮诱导损伤ESCs细胞抗凋亡Bcl-2的mRNA和蛋白的表达（均P<0.000 1），并下调促凋亡蛋白Bax和Caspase-3的mRNA和蛋白的表达（均P<0.000 1）;VEGF、PDGF-BB、TGF-β1抑制剂（Tanshinone IIA、Methylnissolin、CJJ300）均显著逆转PRF-提取液对米非司酮诱导损伤的ESCs细胞的修复作用（均P<0.05）。结论 PRF-提取液可有效修复米非司酮诱导损伤的ESCs细胞，促进ESCs细胞增殖和迁移并调控细胞凋亡蛋白表达，VEGF、PDGF-BB、TGF-β1等细胞因子均参与该作用。
Abstract：Objective To investigate the biological reparative effects of platelet-rich fibrin(PRF) extract on injured endometrial stromal cells(ESCs) induced by mifepristone and the underlying molecular mechanisms.Methods An ESCs injury model was established using mifepristone treatment. Cells were divided into four groups: untreated control(Con), mifepristone-injured group(M), mifepristone treatment+PRF extract group(M+P), and mifepristone treatment+PRF extract+growth factor inhibitors treatment group, including M+P+T, M+P+Me, and M+P+CJ. Cell viability was detected using the CCK-8 assay, and migration ability was evaluated via the scratch wound healing assay. The release kinetics of growth factors, including vascular endothelial growth factor(VEGF), platelet-derived growth factor-BB(PDGF-BB), and transforming growth factor-beta 1(TGF-β1) from PRF extract were measured by ELISA. The mRNA and protein expression levels of apoptosis-related factors(Bcl-2, Bax, and Caspase-3) were analyzed by RT-qPCR and Western blot, respectively.Results VEGF, PDGF-BB, and TGF-β1 were continuously released from the PRF extract over two weeks, with peak release observed on days 3-7. The extract significantly restored the proliferation and migration of mifepristone-injured ESCs(all P<0. 000 1). PRF extract up-regulated the mRNA and protein expression of the anti-apoptotic factor Bcl-2(all P<0. 000 1) and downregulated that of the pro-apoptotic factors Bax and Caspase-3(all P<0. 000 1) in mifepristone-injured ESCs. Inhibitors of VEGF, PDGF-BB, and TGF-β1(Tanshinone IIA, Methylnissolin, and CJJ300) significantly reversed the protective effects of PRF extract on mifepristone-injured ESCs(all P<0. 05).Conclusion PRF extract promotes the repair of injured ESCs by enhancing cell proliferation, attenuating apoptosis, and increasing migration capacity, which involve multiple growth factors, including VEGF, PDGF-BB, and TGF-β1.
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基本信息:
DOI：10.19405/j.cnki.issn1000-1492.2026.08.005
中图分类号:R711.74
引用信息:
[1]邱萍,唐静,刘宇,等.富血小板纤维蛋白提取液对米非司酮诱导损伤的子宫内膜间质细胞的修复作用及其机制研究[J].安徽医科大学学报,2026,61(08):1364-1374.DOI:10.19405/j.cnki.issn1000-1492.2026.08.005.
基金信息:
安徽省卫生健康科研项目（编号：AHWJ2024Ab0039）~~
2026-07-10
2026-07-10
2026-07-10