2024 08 v.59 1417-1422
同源细胞微移植技术联合A型肉毒毒素促进毛发再生的实验研究
基金项目(Foundation):
安徽高校省级自然科学研究重点项目(编号：KJ2014A108)
邮箱(Email):
hfzfzx@163.com;
DOI:
10.19405/j.cnki.issn1000-1492.2024.08.019
中文作者单位:
安徽医科大学第一附属医院整形外科;
摘要(Abstract):
目的 探索同源细胞微移植(MG)技术及A型肉毒毒素(BTX-A)联合使用对小鼠毛发再生的影响。方法 选取45只C57BL/6小鼠，随机分为生理盐水组(NS组)、MG组(MB-0组)、MG+低剂量BTX-A组(MB-2组)、MG+中剂量BTX-A组(MB-10组)、MG+高剂量BTX-A组(MB-50组),共5组，用药后观察小鼠背部毛发生长情况，并在实验第7、14、21天采用苏木精-伊红(HE)染色评估毛囊生长情况、在第21天采用免疫组织化学染色评估血管内皮生长因子(VEGF)及β-catenin的表达情况。结果 经过MG及BTX-A治疗后，小鼠皮肤变黑时间缩短(P<0.05),第14天新生毛发覆盖率增高(P<0.05)。与NS组比较，其余各实验组毛囊数目增多(P<0.05),VEGF及β-catenin表达增多。结论 MG联合BTX-A对小鼠毛发再生有促进作用，其机制可能和促进血管生长及激活β-catenin信号有关。
关键词(KeyWords):
毛囊;;毛发生长;;A型肉毒毒素;;微移植
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参考文献
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[2] Ruiz R G,Rosell J M C,Ceccarelli G,et al.Progenitor-cell-enriched micrografts as a novel option for the management of androgenetic alopecia[J].J Cell Physiol,2020,235(5):4587-93.
[3] Gentile P,Scioli M G,Bielli A,et al.Stem cells from human hair follicles:first mechanical isolation for immediate autologous clinical use in androgenetic alopecia and hair loss[J].Stem Cell Investig,2017,4(7):58.
[4] 李俊兰，温矩.A型肉毒毒素注射治疗雄激素源性脱发的机制及研究进展[J].哈尔滨医药，2023,43(3):137-9.
[5] 景林，侯丽，彭春桥，等.生发小鼠模型建立常用脱毛方法的系统比较[J].中药药理与临床，2017,33(4):205-9.
[6] Li Y,Sakamoto M,Sawaragi E,et al.Comparison of wound healing effect of skin micrograft impregnated into two kinds of artificial dermis in a murine wound model[J].Plast Reconstr Surg Glob Open,2022,10(11):e4636.
[7] Li K N,Tumbar T.Hair follicle stem cells as a skin-organizing signaling center during adult homeostasis[J].EMBO J,2021,40(11):e107135.
[8] 曹可心，张甘霖，杨国旺，等.血余蛋黄油对C57BL/6小鼠毛发生长的影响[J].中华中医药杂志，2017,32(7):3273-5.
[9] 徐林刚，陈龙金，李彦锋，等.自体浓缩生长因子联合A型肉毒毒素治疗男性雄激素性脱发临床疗效[J].中国医疗美容，2022,12(7):51-6.
[10] Ten B,Kaya T ■,Balc? Y,et al.The place of B-mode ultrasonography,shear-wave elastography,and superb microvascular imaging in the pre-diagnosis of androgenetic alopecia[J].J Cosmet Dermatol,2022,21(7):2962-70.
[11] Tian K,Gao S,Jia Z,et al.A study of combination unilateral subcutaneous botulinum toxin a treatment for androgenetic alopecia[J].J Cosmet Dermatol,2022,21(11):5584-90.
[12] Gentile P,Garcovich S.Systematic review of platelet-rich plasma use in androgenetic alopecia compared with minoxidil?,finasteride?,and adult stem cell-based therapy[J].Int J Mol Sci,2020,21(8):2702.
[13] Yuan A R,Bian Q,Gao J Q.Current advances in stem cell-based therapies for hair regeneration[J].Eur J Pharmacol,2020,881:173197.
[14] Jung M K,Song S B,Cheon S Y,et al.Botulinum toxin enhances the implantation effect of adipocytes in C57/BL6 mice[J].Aesth Plast Surg,2009,33(5):722-9.
[15] Liu B,Chen X,Yi H,et al.β-catenin is involved in oleanolic acid-dependent promotion of proliferation in human hair matrix cells in an in vitro organ culture model[J].Fitoterapia,2017,121:136-40.
基本信息:
DOI：10.19405/j.cnki.issn1000-1492.2024.08.019
中图分类号:R758.71
引用信息:
[1]朱秋璇,宋采滢,程盛荣等.同源细胞微移植技术联合A型肉毒毒素促进毛发再生的实验研究[J].安徽医科大学学报,2024,59(08):1417-1422.DOI:10.19405/j.cnki.issn1000-1492.2024.08.019.
基金信息:
安徽高校省级自然科学研究重点项目(编号：KJ2014A108)
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