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目的 探究聚苯乙烯微塑料(PS-MPs)暴露致雄性小鼠血管内皮损伤及木犀草素对血管重塑的干预作用。本研究通过氧化系统和代谢组学探讨其机制。方法 将32只C57BL/6小鼠(6～8周龄)随机分为生理盐水组(saline组)、0.1 mg/kg PSMPs暴露组(0.1PS-MPs组)、1 mg/kg PS-MPs暴露组(1PS-MPs组)、1 mg/kg PS-MPs+木犀草素治疗组(1PS-MPs+Lut组)，每组8只小鼠，经过8周干预后，通过检测各组小鼠体质量、血压、主动脉脏器系数、主动脉组织病理学改变；检测小鼠主动脉中总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)等脂质代谢相关指标；检测活性氧(ROS)、谷胱甘肽(GSH)、丙二醛(MDA)等氧化应激相关指标；检测内皮素(ET-1)、一氧化氮(NO)、血管内皮生长因子A(VEGF-A)、血管细胞黏附分子-1(VCAM-1/CD106)、细胞间黏附分子-1(ICAM-1/CD54)等内皮功能相关指标，以及血清代谢组学。结果 与saline组相比，PSMPs暴露导致小鼠主动脉病理性增厚、主动脉脏器系数增加、血压升高；脂质代谢相关指标TC、TG升高，HDL-C下降，小鼠脂质代谢紊乱；氧化应激相关指标ROS、MDA升高，GSH下降，小鼠体内出现氧化损伤；血管内皮炎症与损伤标志物ET-1、VEGF-A、VCAM-1、ICAM-1水平升高，舒血管物质NO下降，小鼠血管内皮损伤。此外，血清代谢组学结果显示，PS-MPs暴露通过干扰柠檬酸循环等代谢途径导致内皮损伤。相较于1PS-MPs组，木犀草素显著逆转这些效应，降低小鼠体内氧化应激，改善脂质代谢紊乱，有效修复内皮损伤。结论 PS-MPs通过氧化应激和脂质代谢诱导血管毒性，木犀草素有效缓解内皮损伤和血管重塑。
Abstract：Objective To explore the vascular endothelial injury in male mice caused by exposure to polystyrene microplastics(PS-MPs) and the intervention effect of luteolin on vascular remodeling.Additionally, to investigate the mechanism through the oxidative system and metabolomics.Methods Thirty-two C57 BL/6 mice(6-8 weeks old) were randomly divided into the saline group(saline group), the 0.1 mg/kg PS-MPs exposure group(0.1 PSMPs group), the 1 mg/kg PS-MPs exposure group(1 PS-MPs group), and the 1 mg/kg PS-MPs + luteolin treatment group(1 PS-MPs + Lut group), with 8 mice in each group.After 8 weeks of intervention, the body weight, blood pressure, aortic organ coefficient, and aortic histopathological changes of mice in each group were detected; the to‑tal cholesterol(TC), triglyceride(TG), and high-density lipoprotein cholesterol(HDL-C) lipid metabolismrelated indicators in the aorta of mice were detected; the reactive oxygen species(ROS), glutathione(GSH), and malondialdehyde(MDA) oxidative stress-related indicators were detected; the endothelin(ET-1), nitric oxide(NO), vascular endothelial growth factor A(VEGF-A), vascular cell adhesion molecule-1(VCAM-1/CD106), and intercellular adhesion molecule-1(ICAM-1/CD54) endothelial function-related indicators and serum metabolo‑mics were detected.Results Compared to the saline group, exposure to PS-MPs resulted in pathological thicken‑ing of the mouse aorta, increased aortic organ coefficient, and elevated blood pressure.Lipid metabolism-related indicators, including TC and TG, were elevated, while HDL-C was reduced, indicating lipid metabolism disorder in mice.Oxidative stress markers such as ROS and MDA increased, whereas GSH decreased, demonstrating oxida‑tive damage.Vascular endothelial inflammation and injury markers, including ET-1, VEGF-A, VCAM-1, and ICAM-1, were upregulated, while the vasodilatory substance NO was downregulated, confirming endothelial in‑jury.Furthermore, serum metabolomics results revealed that PS-MPs exposure induced endothelial damage by dis‑rupting metabolic pathways such as the citrate cycle.Compared to the PS-MPs group, luteolin significantly re‑versed these effects, attenuating oxidative stress and lipid metabolism disorders, and effectively repairing endothe‑lial injury.Conclusion PS-MPs induce vascular toxicity through oxidative stress and lipid metabolism.Luteolin effectively alleviates endothelial damage and vascular remodeling.
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基本信息:
DOI：10.19405/j.cnki.issn1000-1492.2026.03.007
中图分类号:R994.6
引用信息:
[1]朱德育,黄琪,梁潇,等.木犀草素防治聚苯乙烯微塑料诱导的血管损伤机制研究[J].安徽医科大学学报,2026,61(03):432-438.DOI:10.19405/j.cnki.issn1000-1492.2026.03.007.
基金信息:
国家自然科学基金项目（编号：42177416）; 湖北科技学院医学科研专项重点项目（编号：2022YKY01）; 湖北省咸宁市科技计划社发类研发重点专项（编号：2023SFYF095）; 湖北省自然科学基金咸宁创新发展联合基金重点项目（编号：2025AFD389）~~
2026-02-09
2026-02-09
2026-02-09