安徽医科大学第一附属医院普外科;安徽医科大学第一临床学院;
目的 制备负载大蒜新素——二烯丙基三硫化物(DATS)的中空介孔硅纳米微球(HMSNs),并研究其作为下肢缺血性损伤治疗剂的可行性。方法 采用选择性蚀刻法合成出HMSNs,同时利用扫描和透射电镜观察微观结构，X射线衍射和动态光散射(DLS)分析理化性质，红细胞溶血实验和细胞毒性实验测试生物安全性。采用吸附法将DATS负载至HMSNs里，获得缓释DATS的纳米颗粒(DATS-HMSNs),并用紫外分光光度法计算并制作DATS的累积释放曲线。将C57BL/6小鼠随机分为四组(假手术组、0.9%氯化钠注射液组、DATS组、DATS-HMSNs组),下肢缺血模型采用股动脉结扎切除法制作。测试各组小鼠肢体缺血前后的运动能力和肌肉内肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、单核细胞趋化蛋-1 (MCP-1)、活性氧(ROS)、血小板内皮细胞黏附分子(CD31)、α-平滑肌肌动蛋白(α-SMA)、碱性成纤维细胞生长因子(bFGF)和血管内皮生长因子(VEGF)含量的变化。结果 扫描和透射电镜观察可见制备出的HMSNs为中空的球形且粒径均一，DLS结果显示粒径为(226.5±11.8)nm。红细胞溶血实验及细胞毒性实验结果表明HMSNs具备良好的生物相容性。HMSNs对DATS的最大载药率为27.89%,7 d DATS的累积释放率80.12%,21 d可达到97.27%。与对照组比较，DATS-HMSNs用于下肢缺血小鼠后，免疫组化染色发现CD31、α-SMA、bFGF及VEGF的水平增高(P<0.05),ELISA实验发现TNF-α、IL-6、MCP-1和ROS的含量减低(P<0.05),并且缺血后小鼠运动能力恢复满意。结论 DATS-HMSNs可以缓慢、持续地释放DATS,为下肢缺血性损伤提供保护作用。
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基本信息:
DOI：10.19405/j.cnki.issn1000-1492.2025.02.003
中图分类号:R543;R943
引用信息:
[1]欧阳欢,刘博,刘逸等.大蒜新素纳米缓释颗粒的制备及其对下肢缺血保护作用的实验研究[J].安徽医科大学学报,2025,60(02):201-209.DOI:10.19405/j.cnki.issn1000-1492.2025.02.003.
基金信息:
安徽省高等学校自然科学研究项目(编号：KJ2020A0195)~~